It happened this morning in Sweet Home along Harper Road, according to the Pulaski County Sheriff's Office (PCSO).
The train tracks run alongside Highway 365. Deputies say the impact pushed the vehicle 500 yards down the tracks.
The train was stopped on the tracks as crews tended to the victims in the vehicle.
The PCSO says a juvenile boy died at the scene. The injured victims are described only as a man, a teen boy and a juvenile girl. The girl was taken away from the scene by Med-Flight. The man reportedly had injuries to his arms and legs and the teen boy suffered back injuries.
No other details have been released about the condition of victims or the vehicle in which they were traveling.